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Registros recuperados: 273 | |
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Prudence, Marie; Moal, Jeanne; Boudry, Pierre; Daniel, Jean-yves; Quere, Claudie; Jeffroy, Fanny; Mingant, Christian; Ropert, Michel; Bedier, Edouard; Van Wormhoudt, A; Samain, Jean-francois; Huvet, Arnaud. |
This study investigated the non-neutrality of genetic polymorphism in two alpha-amylase genes (AMYA and AMYB) in the oyster Crassostrea gigas. Bi-parental oyster families, bred to be polymorphic for markers in these genes, were monitored for growth and survival for 1 year under standard culture conditions in two French production sites. Within-family genotype frequencies indicated that the two amylase genes were closely linked (c. 1.7 cM). Within two of three families, significant differences in growth were observed between genotypes at one of the two production sites, suggesting that this polymorphism is not neutral and might be under selection because of its role in digestive function. Estimated daily yields were different between amylase genotypes,... |
Tipo: Text |
Palavras-chave: Growth; Genotype; Digestive enzyme; Bivalve; Alpha amylase. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1691.pdf |
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Gagnaire, Pierre-alexandre; Lamy, Jean-baptiste; Cornette, Florence; Heurtebise, Serge; Degremont, Lionel; Flahauw, Emilie; Boudry, Pierre; Bierne, Nicolas; Lapegue, Sylvie. |
The Pacific cupped oyster is genetically subdivided into two sister taxa, Crassostrea gigas and C. angulata, which are in contact in the north-western Pacific. The nature and origin of their genetic and taxonomic differentiation remains controversial due the lack of known reproductive barriers and the high degree of morphologic similarity. In particular, whether the presence of ecological and/or intrinsic isolating mechanisms contributes to species divergence is unknown. The recent co-introduction of both taxa into Europe offers a unique opportunity to test how genetic differentiation is maintained under new environmental and demographic conditions. We generated a pseudo-chromosome assembly of the Pacific oyster genome using a combination of BAC-end... |
Tipo: Text |
Palavras-chave: Cupped oysters; Genome assembly; Species divergence; Reproductive barriers; Recombination rate. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00455/56622/58337.pdf |
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Boudry, Pierre; Leitao, Alexandra; Mccombie, Helen; Thiriot-quievreux, Catherine. |
Cytogenetic abnormalities arising both in mitosis and meiosis are known to be common in bivalves. Here we review result obtained from the observations of somatic aneuploidy in different populations of the commercially important Pacific oyster, Crassostrea gigas. The oysters studied were either (1) produced in IFREMER hatcheries between 1986 and 1999, (some of these were part of the EU funded programme GENEPHYS); or (2) collected from wild populations along the French Atlantic coast. Chromosome numbers were scored from 30 mitotic metaphases in gill tissue per individual studied. Aneuploid cells (2n - X) were observed in all the 13 populations studied. A highly significant negative correlation was observed between the level of aneuploidy and growth, i.e.... |
Tipo: Text |
Palavras-chave: Growth; Genetic; Aneuploidy; Crassostrea gigas; Pacific oysters. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/acte-3446.pdf |
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Roussel, Sabine; Bisch, T; Lachambre, S; Boudry, Pierre; Gervois, Jl; Lambert, Christophe; Huchette, S; Day, R. |
Domestication of Haliotis tuberculata has only recently begun. During the process, we expect that behavioural and physiological traits may evolve to become more adapted to their captive environment. These modifications may result from intentional selection of production traits or unconscious and unintentional selection due to conditions experienced in the farm environment. To study this process at the earliest stage, the progeny of 3 different broodstocks obtained from wild parents, selected farmed abalone and randomly sampled farmed abalone, were studied. After rearing for 16 mo in separate tanks, offspring from the 3 progenies were placed together in sea cages at the same density. After 3 yr, behavioural traits were studied, and the immune status after a... |
Tipo: Text |
Palavras-chave: Predation; Hiding behaviour; Haliotis tuberculata; Domestication; Selection; Captive; Abalone. |
Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00487/59841/62989.pdf |
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Lapegue, Sylvie; Bedier, Edouard; Goyard, Emmanuel; Degremont, Lionel; Baud, Jean-pierre; Gerard, Andre; Goulletquer, Philippe; Boudry, Pierre. |
Two oyster species are currently present along the French coasts : the indigenous European flat oyster (Ostrea edulis), and the Pacific cupped oyster (Crassostrea gigas), that has been introduced from Japan since the beginning of the 70ies. The flat oyster successively suffered from two protozoan diseases during the 60ies and its production decreased from 20 000 tons/year by that time to 1 500 tons/year nowadays. Consequently, the oyster production is principally (99%) based upon the Pacific oyster species with approximately 150 000 tons/year among which 90% are grown from the natural spat. However, the hatchery production of this species is developing and was estimated to 400 to 800 millions spat in 2002. Moreover, strengthened relationships between... |
Tipo: Text |
Palavras-chave: Growth; Selection; Genetic breeding; Genetic; Ostrea edulis; Crassostrea gigas; Oysters; Croissance; Sélection; Amélioration génétique; Génétique; Ostrea edulis; Crassostrea gigas; Huîtres. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3491.pdf |
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Boudry, Pierre; Launey, Sophie; Huvet, Arnaud. |
The two common oyster species cultivated in Europe are the flat oyster Ostrea edulis and the cupped oyster Crassostrea gigas. These species have two really different reproduction strategies (oviparity versus larviparity) and histories (natural distribution versus introductions linked to oyster culture). We used microsatellite markers to compare the observed variabilities Europe wide. O. edulis presents a lower rate of alleles per locus than C. gigas. The observed structuration levels (global Fst and by pairs values) are similar for both species, but the spatial organisation of the diversity is different. In O. edulis, appears a differentiation based on the distance (concordance of the results with those obtained with the allozymes) whereas in C. gigas,... |
Tipo: Text |
Palavras-chave: Différenciation génétique; Variabilité; Génétique; Crassostrea gigas; Ostrea edulis; Huîtres. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/acte-3455.pdf |
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Huvet, Arnaud; Jeffroy, F; Fabioux, C; Daniel, Jean-yves; Quillien, Virgile; Van Wormhoudt, A; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre; Pouvreau, Stephane. |
To examine further a previously reported association between amylase gene polymorphism and growth in the Pacific oyster Crassostrea gigas, ecophysiological parameters and biochemical and molecular expression levels of alpha-amylase were studied in Pacific oysters of different amylase genotypes. Genotypes that previously displayed significantly different growth were found to be significantly different for ingestion and absorption efficiency. These estimated parameters, used in a dynamic energy budget model, showed that observed ingestion rates (unlike absorption efficiencies) allowed an accurate prediction of growth potential in these genotypes. The observed association between growth and amylase gene polymorphism is therefore more likely to be related to... |
Tipo: Text |
Palavras-chave: Ingestion; Gene expression; Bivalve; Alpha amylase. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-5165.pdf |
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Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc. |
Fertilization of the oocyte is a critical process of sexual reproduction depending among other factors on spermatozoa functionality. Mitochondria participate in many crucial processes for spermatozoa motility and fertilizing ability. The Pacific oyster, Crassostrea gigas, is characterized by a high inter-individual variability of its reproductive success which has been shown to be partially due to the variability of gamete quality. The present study explored spermatozoa characteristics such as: i) relationships among mitochondrial functionality (ATP content, mitochondrial membrane potential (MMP), reactive oxygen species production); sperm viability (percentages of live, dying, and dead); and motility; and ii) their involvement in inter-oyster variability... |
Tipo: Text |
Palavras-chave: Spermatozoa; Quality; Motility; Viability; ATP content; ROS production. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00386/49769/50309.pdf |
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Ernande, Bruno; Boudry, Pierre; Heurtebise, Serge; Haure, Joel; Martin, Jean-louis. |
Growth and survival are the two most interesting characters for oyster farming as they determine the biomass produced in the farms (growth x survival). Thus, a selection program aiming at improving these two characters could be of a great help. However, the oyster, as a sessile organism, is influenced by environmental variability (spatial and temporal) either natural or caused by the used oyster culture methods. Submitted to this variability, oysters must have evolved so as to adapt (short term) to this environmental variability. |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Huîtres; Croissance; Plasticité; Génétique. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/acte-3279.pdf |
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Baud, Jean-pierre; Palvadeau, Hubert; Nourry, Max; Haure, Joel; Penisson, Christian; Boudry, Pierre. |
La production française d' huître creuse Crassostrea gigas représente, avec 144000 tonnes de production, la 4ème' place au ni veau mondial après la Chine (2 285 000 t), le Japon (225 000t.) et la Corée (1 85 000t.). Ce bivalve filtreur possède un potentiel d'adaptation élevé puisqu'on le trouve sous des latitudes très diverses. Malgré son importance économique mondiale (2 900 000 tonnes de production en 1996), à ce jour, très peu de programmes de sélection ont été mis en oeuvre sur cette espèce, qui n'est pas considérée comme domestiquées au sens génétique du terme. Toutefois, avant de lancer des programmes - nécessairement de grande ampleur - pour la sélection de souches d' huîtres creuses Crassostrea gigas, il est utile de répondre au préalable à... |
Tipo: Text |
Palavras-chave: Génétique; Variabilité génétique; Croissance; Huîtres creuses; Crassostrea gigas. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00048/15881/13303.pdf |
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Cochennec-laureau, Nathalie; Baud, Jean-pierre; Bedier, Edouard; Boudry, Pierre; Huvet, Arnaud; Nicolas, Jean-louis; Pepin, Jean-francois; Petton, Bruno. |
Ce bilan comprend deux parties : la première est dédiée à une synthèse des travaux présentés lors des journées « Surmortalités des huîtres creuses, Crassostrea gigas » les 8 et 9 décembre 2009 et, la seconde partie, à une proposition d'un projet de recherche spécifique 2010-2012 en complément des propositions de « sortie de crise ». Contexte Les mortalités estivales de l'huître creuse, Crassostrea gigas, sont décrites depuis une vingtaine d'années en France mais également au Japon, en Corée, aux USA et en Australie. Ces phénomènes ou « syndrome des mortalités estivales » ont fait l'objet d'une étude pluridisciplinaire en France entre 2001 et 2006 (Défi MOREST). Toutefois, en 2008 et 2009, l'ostréiculture française a du faire face à deux épisodes de... |
Tipo: Text |
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Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/2010/acte-7393.pdf |
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Bierne, Nicolas; Boudry, Pierre; Lapegue, Sylvie; Bonhomme, Francois; Faure, M; Sauvage, Christopher; Moraga, D; Boutet, Isabelle; David, Elise; Jollivet, Didier; Tanguy, Arnaud; Faure, Baptiste; David, Patrice. |
For a long time, population geneticists have had to content themselves with analysing neutral markers to infer selection processes indirectly. The recent improvement of sequencing tools now enables them to analyse the variations of a large number of genes and therefore to spot the genes, or the amino acids, which are under the direct influence of the selection (Yang & Bielawski, 2000). In order to analyse the selection processes acting on the mutations and discriminate the different factors acting on their evolution (genetic selection or drift), it is essential to compare polymorphism data between genomes of the same species/population and divergence data between species/populations (Kimura, 1983). Recently, polymorphism data has become available for a... |
Tipo: Text |
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Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/rapport-6489.pdf |
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Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre. |
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,... |
Tipo: Text |
Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf |
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Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre. |
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production. Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-Like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea.... |
Tipo: Text |
Palavras-chave: Crassostrea rivularis; Suminoe oyster; Bonamiosis; Bonamia-like parasite. |
Ano: 1998 |
URL: https://archimer.ifremer.fr/doc/00446/55795/62652.pdf |
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Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumont, Andrew; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in the aquaculture production of this oyster species. Therefore a selective breeding program for resistance to bonamiosis has been undertaken since 1985 bu Ifremer, leading to the production of several selected oyster families. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters (injected with the parasite) to two tested families of oysters originating from the selective breeding program. Mortalities were checked daily, and ventricular heart smears were performed on dying or moribund oysters to detect the level of infection to... |
Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4535.pdf |
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Degremont, Lionel; Fortin, Adeline; Boudry, Pierre. |
Dans le cadre du programme MOREST, une première génération d'huître creuse Crassostrea gigas (G1) a été réalisée en 2001 à partir d'un plan de croisement hiérarchisé par les mâles. La caractérisation sur estran de 44 familles de plein-frères réparties en 17 familles de demi-frères a permis d'estimer l'héritabilité de la survie des naissains en période estivale. La valeur obtenue (h2 = 0,81 ± 0,29) indique que la majorité de la variance phénotypique est d'origine génétique. A partir de familles G1 sélectionnées pour les performances de survies, une sélection divergente visant à améliorer ou diminuer la survie du naissain en période estivale a été réalisée en 2002 et constitue la seconde génération (G2). La sélection divergente comprend deux séries de... |
Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Génétique; Sélection; Estran. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00035/14668/11970.pdf |
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Delaporte, Maryse; Soudant, P; Lambert, C; Jegaden, M; Moal, Jeanne; Pouvreau, Stephane; Degremont, Lionel; Boudry, Pierre; Samain, Jean-francois. |
Within the framework of a national scientific program named "MORtalites ESTivales de l'huitre creuse Crassostrea gigas" (MOREST), a family-based experiment was developed to study the genetic basis of resistance to summer mortality in the Pacific oyster, Crassostrea gigas. As part of the MOREST project, the second generation of three resistant families and two susceptible families were chosen and pooled into two respective groups: "R" and "S". These two groups of oysters were conditioned for 6 months on two food levels (4% and 12% of oyster soft-tissue dry weight in algal dry weight per day) with a temperature gradient that mimicked the Marennes-Oleron natural cycle during the oyster reproductive period. Oyster mortality remained low for the first two... |
Tipo: Text |
Palavras-chave: Summer mortality; Reproduction; Reactive oxygen species ROS; Hemocyte parameters; Genetic selection; Crassostrea gigas. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3578.pdf |
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Registros recuperados: 273 | |
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